Biocortech S.A.S.

RNA extracts R from cellular

• Jesse
• November 27, 2021
• 0

RNase R shows low train on tRNA, rRNA and completely different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use further RNase R,

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the look at of lariat RNA produced by typical splicing, along with circRNAs which come up via back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species via RNA-sequencing. This allows researchers to probe the panorama of splicing events with greater depth.

APPLICATIONS:

• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying numerous splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all elements to retain most effectivity. All elements
are regular for one 12 months from the date of transport when saved and handled accurately.

 

Cat # +Dimension	M1228-500
Dimension	500 U
Highlights	RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions	-20°C
Supply Conditions	Gel Pack
USAGE	For Evaluation Use Solely! Not For Use in Individuals.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are typically required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the method. The higher temperature is very useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this can end in non-enzymatic RNA degradation.

•
or take away rRNA from full RNA extracts earlier to digestion.

• Do not forget that spherical RNAs symbolize a small proportion of full RNA (typically 0.1%-0.01%), as a result of this truth RNase R treatment will most definitely finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is helpful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be helpful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation may be utilized as an alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is helpful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

RELATED PRODUCTS:

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

 

Histone H3 Peptide (residues 21-44), Biotinylated
R-1006	EpiGentek	EUR 362.76 EUR 119.90 EUR 205.70	200 µl 100 ul 200 ul

Histone H4 Peptide (residues 1-21), Biotinylated
R-1007	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H4ac Peptide, Biotinylated
R-1008	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide
R-1009	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide, Biotinylated
R-1010	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide
R-1011	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide, Biotinylated
R-1012	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H4K5ac Peptide
R-1014	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K8ac Peptide
R-1015	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K12ac Peptide
R-1016	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K16ac Peptide
R-1017	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide
R-1018	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide, Biotinylated
R-1019	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide
R-1020	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide, Biotinylated
R-1021	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide
R-1022	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide, Biotinylated
R-1023	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide
R-1024	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide, Biotinylated
R-1025	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide
R-1026	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated
R-1027	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide
R-1028	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated
R-1029	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide
R-1030	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated
R-1031	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me2 Dimethyl Peptide
R-1032	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me2 Dimethyl Peptide, Biotinylated
R-1033	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me3 Trimethyl Peptide
R-1034	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me3 Trimethyl Peptide, Biotinylated
R-1035	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K36me1 Monomethyl Peptide
R-1036	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K36me1 Monomethyl Peptide, Biotinylated
R-1037	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K36me2 Dimethyl Peptide
R-1038	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K36me3 Trimethyl Peptide
R-1039	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul