Biocortech S.A.S.

nucleocapsid phosphoprotein Phire and Phusion Kits

• Jesse
• November 27, 2021
• 0

The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification know-how for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who could or might not be suspected of getting COVID-19.

Unit Definition

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description	Code	Specification	Class	Expertise
ALS SARS-CoV-2 RT-PCR Take a look at for surfaces	ALS-B01-Adv	RUO	Meals /Water Virology Detection	Actual-Time PCR
ALS SARS-CoV-2 RT-PCR Take a look at for Wastewater	ALS-B02-Adv	RUO	Meals /Water Virology Detection	Actual-Time PCR

 

Benefits

• Fast amplification: The amplification fee of the polymerase is 6kb/min. 1kb fragment will be amplified inside 25min.
• Lengthy fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
• Excessive specificity: With scorching begin know-how, the polymerase is 100% inactive beneath 50°C, and might solely be restored by heating at 95°C for 5min.
• Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine will be straight amplified with out prior DNA purification.

What’s Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification straight from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system drastically reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.

 

In some ways, dPCR works like standard PCR. Each methods depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors usually present in crude samples.

 

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all identified SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different identified associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been primarily based on WHO, CDC and DGS suggestions.

 

The equipment makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment can even detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.

 

 

Direct PCR Complete Kit
S700	GeneOn	100 rcs	EUR 159.6

Direct PCR Complete Kit
S700-2	GeneOn	5x100 rcs	EUR 628.8

Direct Blood PCR Kit
E0950-01	EURx	100reactions	EUR 69.76
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit
E0950-02	EURx	500reactions	EUR 277.95
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Plant PCR Kit
E0960-01	EURx	100reactions	EUR 55.59
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Plant PCR Kit
E0960-02	EURx	500reactions	EUR 251.79
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Blood PCR Kit
MBPCR171-100R	EWC Diagnostics	1 unit	EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit
MBPCR171-10R	EWC Diagnostics	1 unit	EUR 71.73
Description: Direct Blood PCR Kit

Direct Tissue PCR Kit
E0940-01	EURx	100reactions	EUR 55.59
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Direct Tissue PCR Kit
E0940-02	EURx	500reactions	EUR 251.79
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Blood Direct PCR Kit V2
PD103-01	Vazyme	50 rxn	EUR 51.41

Blood Direct PCR Kit V2
PD103-02	Vazyme	200 rxn	EUR 188.5

EzWay Plant Direct PCR Kit
K0568700	KOMA BIOTECH	1set	EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568700.pdf

Mouse tissue direct PCR kit
EP033	ELK Biotech	50T	EUR 125

Mouse tissue direct PCR kit
EP033-200T	ELK Biotech	200T	EUR 125

Mouse tissue direct PCR kit
EP033-50T	ELK Biotech	50T	EUR 40

Mouse Tissue Direct PCR Kit
RK20736	Abclonal	100RXN	EUR 149.88

amfiSure Direct PCR Lysis Buffer
D0300-010	GenDepot	100ml	EUR 165.6

FastAmp® Plant Direct PCR Kit
4615	Intact Genomics	1/EA	EUR 396
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water

EzWay Mouse Tail Direct PCR Kit
K0568600	KOMA BIOTECH	1set	EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568600.pdf

Mouse Direct PCR Kit (For Genotyping)
B45012	Bimake	1ml*5 (500 ractions)	EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B45013	Bimake	1ml*20 (2000 ractions)	EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40013	Bimake	200 ractions	EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)
B40015	Bimake	500 ractions	EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Tissue Direct PCR Kit (With Dye)
10185ES50	Yeasen Biotechnology	50T	EUR 65

Mouse Tissue Direct PCR Kit (With Dye)
10185ES70	Yeasen Biotechnology	200T	EUR 235

EzWay Direct Taq PCR MasterMix (2x)
K0568010	KOMA BIOTECH	1ml	EUR 108
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf

EzWay Direct Hot Taq PCR MasterMix
K0568210	KOMA BIOTECH	1ml	EUR 122
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf

BioLit Animal Tissue Direct PCR Kit (Research)
91210	Sisco Laboratories	50 Preps	EUR 222.28
Description: Part E

EzWay Direct Multiplex PCR MasterMix (2x)
K0567840	KOMA BIOTECH	1ml	EUR 161
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf

FastAmp® Plant Direct PCR & Genotyping Solution
4611	Intact Genomics	20mL	EUR 156
Description: FastAmp® Plant Direct PCR/Genotyping Solution for is designed for rapid cell lysis and DNA separation simultaneously without the need of DNA extraction steps . It contains very low concentration of less toxic chemicals for direct DNA amplification of genomic DNA from various plant tissues. No heat treatment and no termination buffer is is required, saving significant time. FastAmp® Plant Direct PCR/Genotyping Solution has been optimized with FastAmp® Plant Direct PCR kit and FastAmp® Plant Tissue/Seed Genotyping PCR kit.

Allele-In-One Mouse Tail Direct PCR Kit
ABP-PP-MT02100	Allele Biotech	100 RXNS	Ask for price