nucleocapsid phosphoprotein Phire and Phusion Kits
The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification know-how for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who could or might not be suspected of getting COVID-19.
Unit Definition
One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.
| Description | Code | Specification | Class | Expertise |
|---|---|---|---|---|
| ALS SARS-CoV-2 RT-PCR Take a look at for surfaces | ALS-B01-Adv | RUO | Meals /Water Virology Detection | Actual-Time PCR |
| ALS SARS-CoV-2 RT-PCR Take a look at for Wastewater | ALS-B02-Adv | RUO | Meals /Water Virology Detection | Actual-Time PCR |
Benefits
- Fast amplification: The amplification fee of the polymerase is 6kb/min. 1kb fragment will be amplified inside 25min.
- Lengthy fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
- Excessive specificity: With scorching begin know-how, the polymerase is 100% inactive beneath 50°C, and might solely be restored by heating at 95°C for 5min.
- Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine will be straight amplified with out prior DNA purification.
What’s Direct PCR?
Direct PCR (dPCR) is a technique of DNA amplification straight from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system drastically reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.
In some ways, dPCR works like standard PCR. Each methods depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors usually present in crude samples.
ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all identified SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different identified associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been primarily based on WHO, CDC and DGS suggestions.
The equipment makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment can even detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.
Direct PCR Complete Kit |
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| S700 | GeneOn | 100 rcs | EUR 159.6 |
Direct PCR Complete Kit |
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| S700-2 | GeneOn | 5x100 rcs | EUR 628.8 |
Direct Blood PCR Kit |
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| MBPCR171-100R | EWC Diagnostics | 1 unit | EUR 267.52 |
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Description: Direct Blood PCR Kit |
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Direct Blood PCR Kit |
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| MBPCR171-10R | EWC Diagnostics | 1 unit | EUR 71.73 |
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Description: Direct Blood PCR Kit |
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Direct Blood PCR Kit |
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| E0950-01 | EURx | 100reactions | EUR 69.76 |
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Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification |
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Direct Blood PCR Kit |
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| E0950-02 | EURx | 500reactions | EUR 277.95 |
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Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification |
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Direct Plant PCR Kit |
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| E0960-01 | EURx | 100reactions | EUR 55.59 |
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Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification |
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Direct Plant PCR Kit |
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| E0960-02 | EURx | 500reactions | EUR 251.79 |
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Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification |
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Direct Tissue PCR Kit |
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| E0940-01 | EURx | 100reactions | EUR 55.59 |
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Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification |
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Direct Tissue PCR Kit |
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| E0940-02 | EURx | 500reactions | EUR 251.79 |
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Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification |
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Blood Direct PCR Kit V2 |
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| PD103-01 | Vazyme | 50 rxn | EUR 51.41 |
Blood Direct PCR Kit V2 |
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| PD103-02 | Vazyme | 200 rxn | EUR 188.5 |
Mouse tissue direct PCR kit |
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| EP033-200T | ELK Biotech | 200T | EUR 125 |
Mouse tissue direct PCR kit |
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| EP033-50T | ELK Biotech | 50T | EUR 40 |
Mouse tissue direct PCR kit |
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| EP033 | ELK Biotech | 50T | EUR 125 |
Mouse Tissue Direct PCR Kit |
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| RK20736 | Abclonal | 100RXN | EUR 150 |
amfiSure Direct PCR Lysis Buffer |
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| D0300-010 | GenDepot | 100ml | EUR 165.6 |
Mouse Direct PCR Kit (For Genotyping) |
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| B40013 | Bimake | 200 ractions | EUR 250.8 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B40015 | Bimake | 500 ractions | EUR 490.8 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B45012 | Bimake | 1ml*5 (500 ractions) | EUR 336 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B45013 | Bimake | 1ml*20 (2000 ractions) | EUR 1038 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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BioLit Animal Tissue Direct PCR Kit (Research) |
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| 91210 | Sisco Laboratories | 50 Preps | EUR 222.28 |
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Description: Part E |
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Allele-In-One Mouse Tail Direct PCR Kit |
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| ABP-PP-MT02100 | Allele Biotech | 100 RXNS | Ask for price |
Allele-In-One Mouse Tail Direct PCR Kit |
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| ABP-PP-MT02500 | Allele Biotech | 500 RXNS | Ask for price |
Allele-In-One Human Blood Direct PCR Kit |
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| ABP-PP-BD02100 | Allele Biotech | 100 RXNS | Ask for price |
Allele-In-One Human Blood Direct PCR Kit |
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| ABP-PP-BD02500 | Allele Biotech | 500 RXNS | Ask for price |
FastAmp™ Plant Direct PCR Kit - 1,250 Reactions |
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| 4615 | Intact Genomics | 1/EA | EUR 495 |
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Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water |
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One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD) |
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| 105-540 | GeneOn | 20 rcs x 50 µl | EUR 196.8 |
One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD) |
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| 105-542 | GeneOn | 100 rcs x 50 µl | EUR 658.8 |
One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD) |
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| 105-544 | GeneOn | 1000 rcs x 50 µl | EUR 5026.8 |
FastAmp® Plant Direct PCR & Genotyping Solution - 20mL |
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| 4611 | Intact Genomics | 20mL | EUR 195 |
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Description: FastAmp® Plant Direct PCR/Genotyping Solution for is designed for rapid cell lysis and DNA separation simultaneously without the need of DNA extraction steps . It contains very low concentration of less toxic chemicals for direct DNA amplification of genomic DNA from various plant tissues. No heat treatment and no termination buffer is is required, saving significant time. FastAmp® Plant Direct PCR/Genotyping Solution has been optimized with FastAmp® Plant Direct PCR kit and FastAmp® Plant Tissue/Seed Genotyping PCR kit. |
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Phusion Blood Direct PCR Kit - 500 x 20 µL rxns |
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| THF-547L | Westburg | each | EUR 583.15 |
