• May 22, 2021

Hemoglobin Colorimetric Detection Kit

The Hemoglobin Detection Research Exclusive Use Kit is a colorimetric assay designed for the quantification and detection of hemoglobin in whole blood, red blood cells, and hemolyzed serum and plasma samples.

This complete, ready-to-use kit includes 96-well clear plates, hemoglobin standard, and other components to perform the assay. A 96-well microplate reader capable of reading an optical density between 560 and 580 nm is required to use this kit.

Presentation features

  •  Assay type: colorimetric detection kit
  • Sample types: whole blood, red blood cells, and hemolyzed serum and plasma samples
  • Sensitivity 0.021 g / dL
  • Standard curve range: 0.25 g / dL – 16 g / dL
  • Reactivity: independent species

Background

Hemoglobin (Hgb) is an erythrocyte protein complex made up of two sets of identical pairs of subunits, each of which binds to an iron-porphyrin group commonly called heme. It generally contains two chains of alpha or alpha-type globulin, the remaining subunits can be beta, gamma, delta, or epsilon, or in the case of babies, fetal hemoglobin that is replaced during the first year of life. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension.

Free oxygen or carbon dioxide attached by a heme group facilitates the subsequent attachment of the other heme groups on a given hemoglobin molecule.

Test principle

The Colorimetric Hemoglobin Detection Kit is designed to quantitatively measure all forms of hemoglobin present in blood and red blood cells, or plasma and serum.

A human hemoglobin standard is provided to generate a standard curve for the assay and all samples must be read from the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate and ready-to-use Hemoglobin Detection Reagent is added to each well. For whole blood or red blood cell samples, 10 μL of samples and standards are used in the regular format.

Results are calculated as g/dl for whole blood and red blood cells, and mg/ml for serum and plasma. The plate is incubated for 30 minutes at room temperature. The plate is read at 560-580 nm to detect the intensity of the generated color. This hemoglobin detection kit uses a unique reaction solution that is light stable at 4°C and does not contain hazardous chemicals.

All forms of hemoglobin rapidly convert to a single stable form that is photometrically measured. Many undiluted samples can be measured in this simple and safe assay.

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